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. 2021 May 15;28(11):2991–3008. doi: 10.1038/s41418-021-00796-x

Table 1.

Double-sided interacting pairs.

Combination of death domains ALPHA signal (RLU) Interaction
DFD 1 DFD 2 Homo/hetero VaProS (updated: December 8, 2020) BioGRID (build 4.1.190) Others
No. Name No. Name DFD 1: DFD 2: FLAG Biotin Biotin FLAG Description PubMed ID Interaction confirmed by PubMed ID Description/PubMed ID
001 AIM2_PYD 009 ASC_PYD Homotypic 8602 4522 Association 24630722 N/A AIM2 inflammasome
005 ANKDD1A_DD 029 CASP12_CARD Heterotypic 2058 1148 N/A N/A Not found
005 ANKDD1A_DD 071 NLRC3_CARD Heterotypic 964 3554 N/A N/A Not found
007 APAF1_CARD 036 CASP9_CARD Homotypic 119,174 104,846 Direct interaction 9390557 Affinity Capture-MS 17643375 Apoptosome
008 ASC_CARD 018 CARD16_CARD Homotypic 1972 2184 N/A N/A 25973362
008 ASC_CARD 025 CASP1_CARD Homotypic 1924 11,030 Direct interaction 24630722 Affinity Capture-Western 26121674 Inflammasome
008 ASC_CARD 072 NLRC4_CARD Homotypic 2690 12,412 Association 12646168 Two-hybrid 11374873 NLRC4 inflammasome
010 ASC_PYD-CARD 025 CASP1_CARD Homotypic 1728 4880 Direct interaction 24630722 Affinity Capture-Western 26121674 Inflammasome
010 ASC_PYD-CARD 072 NLRC4_CARD Homotypic 1688 4502 Association 12646168 Two-hybrid 11374873 NLRC4 inflammasome
017 CARD14_CARD 021 CARD19_CARD Homotypic 1834 956 N/A N/A Not found
018 CARD16_CARD 023 CARD8_CARD Homotypic 1090 1434 N/A N/A Not found
018 CARD16_CARD 025 CASP1_CARD Homotypic 14,594 11,976 N/A Affinity Capture-MS 31091453 11432859
018 CARD16_CARD 072 NLRC4_CARD Homotypic 958 13,094 N/A N/A Direct interaction not approved
019 CARD17_CARD 025 CASP1_CARD Homotypic 1516 9228 Direct interaction 27043298 N/A 15383541
020 CARD18_CARD 025 CASP1_CARD Homotypic 1458 12,258 Direct interaction 27043298 N/A 11051551
023 CARD8_CARD 025 CASP1_CARD Homotypic 1610 11,702 N/A N/A 11821383
028 CASP10_DED1-DED2 035 CASP8_DED1-DED2 Homotypic 5312 5596 Association 12887920 Affinity Capture-MS 19615732
028 CASP10_DED1-DED2 039 CFLAR_DED1-DED2 Homotypic 8826 24,620 Association 23541952 Affinity Capture-MS 21303910 23070002
028 CASP10_DED1-DED2 051 FADD_DED Homotypic 6358 31,336 Direct interaction 11717445 Affinity Capture-MS 19615732 9184224
028 CASP10_DED1-DED2 052 FADD_DED-DD 10,204 20,660 Direct interaction 11717445 Affinity Capture-MS 19615732 9184224
030 CASP2_CARD 041 CRADD_CARD-DD 14,156 2416 Association 11156409 Affinity Capture-MS 20562859
033 CASP8_DED1 061 IRAK3_DD Heterotypic 968 1346 N/A N/A 30372424
034 CASP8_DED2 039 CFLAR_DED1-DED2 Homotypic 9886 5338 Protein cleavage 12887920 Affinity Capture-MS 21303910
035 CASP8_DED1-DED2 039 CFLAR_DED1-DED2 Homotypic 12,804 4088 Protein cleavage 12887920 Affinity Capture-MS 21303910
035 CASP8_DED1-DED2 051 FADD_DED Homotypic 6866 6856 Protein cleavage 12887920 Affinity Capture-MS 19615732 9184224
035 CASP8_DED1-DED2 052 FADD_DED-DD 9378 11,018 Protein cleavage 12887920 Affinity Capture-MS 19615732 9184224
039 CFLAR_DED1-DED2 051 FADD_DED Homotypic 2848 20,516 Association 17047155 Affinity Capture-MS 19369198 9184224
039 CFLAR_DED1-DED2 052 FADD_DED-DD 14,072 20,116 Association 17047155 Affinity Capture-MS 19369198 9184224
042 CRADD_DD 058 IRAK1_DD Homotypic 1632 964 N/A N/A Direct interaction not approved
043 DAPK1_DD 097 PYDC2_PYD Heterotypic 3536 970 N/A N/A Not found
050 FADD_DD 103 RIPK1_DD Homotypic 3828 52,618 Association 19524513 Affinity Capture-MS 26186194 9184224
050 FADD_DD 111 TRADD_DD Homotypic 1384 14,500 Association 30561431 Affinity Capture-MS 21145461 9184224
052 FADD_DED-DD 103 RIPK1_DD 20,016 30,588 Association 19524513 Affinity Capture-MS 26186194 8947041
052 FADD_DED-DD 111 TRADD_DD 39,570 31,748 Association 30561431 Affinity Capture-MS 21145461 8565075
052 FADD_DED-DD 108 TNFRSF1A_DD 964 5568 Physical association 30561431 Affinity Capture-Western 8565075
058 IRAK1_DD 059 IRAK2_DD Homotypic 5756 1960 Physical association 10383454 Affinity Capture-MS 26186194
058 IRAK1_DD 061 IRAK4_DD Homotypic 1644 1630 Physical association 12860405 Affinity Capture-MS 26496610
058 IRAK1_DD 067 MYD88_DD Homotypic 4038 2594 Physical association 17567694 Affinity Capture-MS 22623428
058 IRAK1_DD 083 NLRP4_PYD Heterotypic 980 1062 N/A N/A Not found
058 IRAK1_DD 087 NLRP8_PYD Heterotypic 1158 2224 N/A N/A Not found
058 IRAK1_DD 110 TNFRSF25_DD Homotypic 958 976 N/A N/A Direct interaction not approved
061 IRAK4_DD 088 NLRP9_PYD Heterotypic 1176 1072 N/A N/A Not found
065 PYRIN_PYD 083 NLRP4_PYD Homotypic 1280 3860 N/A N/A Not found
065 PYRIN_PYD 088 NLRP9_PYD Homotypic 1064 4656 N/A N/A Not found
067 MYD88_DD 071 NLRC3_CARD Heterotypic 1144 4342 N/A N/A Not found
067 MYD88_DD 091 NOD2_CARD2 Heterotypic 1432 3318 N/A N/A Direct interaction not approved
071 NLRC3_CARD 083 NLRP4_PYD Heterotypic 1674 976 N/A N/A Not found
073 NLRC5_CARD 088 NLRP9_PYD Heterotypic 968 1088 N/A N/A Not found
080 NLRP14_PYD 083 NLRP4_PYD Homotypic 1166 998 N/A N/A Not found
080 NLRP14_PYD 088 NLRP9_PYD Homotypic 1044 970 N/A N/A Not found
083 NLRP4_PYD 088 NLRP9_PYD Homotypic 2468 4868 N/A N/A Not found
103 RIPK1_DD 108 TNFRSF1A_DD Homotypic 3886 11,094 Physical association 16611992 Affinity Capture-MS 21145461 9184224
103 RIPK1_DD 110 TNFRSF25_DD Homotypic 1762 1122 N/A Affinity Capture-MS 26186194
103 RIPK1_DD 111 TRADD_DD Homotypic 6130 3896 Physical association 8612133 Affinity Capture-MS 18655028 9184224
108 TNFRSF1A_DD 110 TNFRSF25_DD Homotypic 13,248 3080 N/A N/A 9184224
108 TNFRSF1A_DD 111 TRADD_DD Homotypic 33,190 43,604 Physical association 7758105 Affinity Capture-MS 21670149 9184224
110 TNFRSF25_DD 111 TRADD_DD Homotypic 19,914 55,078 N/A Affinity Capture-Luminescence 22939624 9184224

Combinations with ALPHA signals higher than 1 × 103 RLU in both FLAG × biotin and biotin × FLAG pairs, including 57 domain–domain pairs (53 protein–protein pairs). The interaction information was obtained from the following databases: VaProS (https://vapros.org/) and BioGRID (https://thebiogrid.org/). Representative references are listed.