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. 2021 May 15;28(11):2991–3008. doi: 10.1038/s41418-021-00796-x

Table 2.

Combinations hinting at possible interactions.

Combination of death domains AlphaScreen signal (RLU) Interaction
Biotin-tagged domain FLAG-tagged domain Homo/hetero VaProS (updated: December 8, 2020) BioGRID (build 4.1.190)
No. Name No. Name Description PubMed ID Interaction confirmed by PubMed ID
003 ANK2_DD 015 CARD10_CARD Heterotypic 3296 N/A N/A
003 ANK2_DD 047 DTHD1_DD Homotypic 11,380 N/A N/A
003 ANK2_DD 111 TRADD_DD Homotypic 2292 N/A N/A
005 ANKDD1A_PYD 042 CRADD_DD Heterotypic 2906 N/A N/A
005 ANKDD1A_PYD 043 DAPK1_DD Heterotypic 2558 N/A N/A
005 ANKDD1A_PYD 058 IRAK1_DD Heterotypic 5272 N/A N/A
005 ANKDD1A_PYD 061 IRAK4_DD Heterotypic 2228 N/A N/A
005 ANKDD1A_PYD 091 NOD2_CARD2 Heterotypic 2254 N/A N/A
005 ANKDD1A_PYD 115 UNC5D_DD Heterotypic 3122 N/A N/A
009 ASC_PYD 011 BCL10_CARD Heterotypic 7302 Association 22267217 N/A
009 ASC_PYD 065 PYRIN_PYD Homotypic 4142 Physical association 25006247 N/A
009 ASC_PYD 076 NLRP10_PYD Homotypic 5626 N/A N/A
009 ASC_PYD 082 NLRP3_PYD Homotypic 3288 Colocalization 24630722 N/A
009 ASC_PYD 096 PYDC1_PYD Homotypic 4236 Colocalization 17178784 N/A
012 BFAR_DED 042 CRADD_DD Heterotypic 2784 N/A N/A
012 BFAR_DED 058 IRAK1_DD Heterotypic 3826 N/A N/A
012 BFAR_DED 061 IRAK4_DD Heterotypic 2286 N/A N/A
012 BFAR_DED 062 LRRD1_DD Heterotypic 2362 N/A N/A
012 BFAR_DED 071 NLRC3_CARD Heterotypic 2740 N/A N/A
012 BFAR_DED 088 NLRP9_PYD Heterotypic 2282 N/A N/A
012 BFAR_DED 115 UNC5D_DD Heterotypic 3026 N/A N/A
016 CARD11_CARD 015 CARD10_CARD Homotypic 4008 N/A N/A
017 CARD14_CARD 058 IRAK1_DD Heterotypic 2032 N/A N/A
018 CARD16_CARD 020 CARD18_CARD Homotypic 2064 N/A N/A
020 CARD18_CARD 072 NLRC4_CARD Homotypic 3806 N/A N/A
021 CARD19_CARD 011 BCL10_CARD Homotypic 2964 Colocalization 15637807 N/A
021 CARD19_CARD 015 CARD10_CARD Homotypic 4128 N/A N/A
021 CARD19_CARD 030 CASP2_CARD Homotypic 2710 N/A N/A
021 CARD19_CARD 052 FADD_DED-DD Heterotypic 7894 N/A N/A
021 CARD19_CARD 064 MAVS_CARD Homotypic 31,768 N/A N/A
021 CARD19_CARD 088 NLRP9_PYD Heterotypic 2092 N/A N/A
021 CARD19_CARD 108 TNFRSF1A_DD Heterotypic 31,120 N/A N/A
023 CARD8_CARD 072 NLRC4_CARD Homotypic 5474 N/A N/A
026 CASP10_DED1 037 CFLAR_DED1 Homotypic 5528 Association 12887920 Affinity Capture-MS 21303910
026 CASP10_DED1 042 CRADD_DD Heterotypic 3234 N/A N/A
026 CASP10_DED1 047 DTHD1_DD Heterotypic 4662 N/A N/A
026 CASP10_DED1 071 NLRC3_CARD Heterotypic 4320 N/A N/A
026 CASP10_DED1 115 UNC5D_DD Heterotypic 2982 N/A N/A
029 CASP12_CARD 011 BCL10_CARD Homotypic 4998 N/A N/A
029 CASP12_CARD 037 CFLAR_DED1 Heterotypic 2042 N/A N/A
029 CASP12_CARD 042 CRADD_DD Heterotypic 3880 N/A N/A
029 CASP12_CARD 044 DEDD_PYD Heterotypic 2244 N/A N/A
029 CASP12_CARD 047 DTHD1_DD Heterotypic 2052 N/A N/A
029 CASP12_CARD 061 IRAK4_DD Heterotypic 2518 N/A N/A
029 CASP12_CARD 062 LRRD1_DD Heterotypic 2330 N/A N/A
029 CASP12_CARD 071 NLRC3_CARD Homotypic 2600 N/A N/A
029 CASP12_CARD 088 NLRP9_PYD Heterotypic 4390 N/A N/A
029 CASP12_CARD 115 UNC5D_DD Heterotypic 2976 N/A N/A
033 CASP8_DED1 058 IRAK1_DD Heterotypic 2774 N/A N/A
033 CASP8_DED1 071 NLRC3_CARD Heterotypic 2390 N/A N/A
034 CASP8_DED2 038 CFLAR_DED2 Homotypic 2734 N/A Affinity Capture-MS 21303910
034 CASP8_DED2 051 FADD_DED Heterotypic 4662 Association 18946037 Affinity Capture-MS 19615732
034 CASP8_DED2 052 FADD_DED-DD 4062 Association 18946037 Affinity Capture-MS 19615732
034 CASP8_DED2 071 NLRC3_CARD Heterotypic 2000 N/A N/A
036 CASP9_CARD 011 BCL10_CARD Homotypic 2182 N/A N/A
036 CASP9_CARD 049 EDARADD_DD Heterotypic 2740 N/A N/A
036 CASP9_CARD 064 MAVS_CARD Homotypic 28,754 N/A N/A
045 DEDD2_DED 044 DEDD_PYD Heterotypic 2556 N/A Affinity Capture-MS 19738201
048 EDAR_DD 011 BCL10_CARD Heterotypic 2040 N/A N/A
050 FADD_DD 108 TNFRSF1A_DD Homotypic 5854 Physical association 16611992 Affinity Capture-MS 19615732
053 FAS_DD 052 FADD_DED-DD 38,730 Physical association 7536190 Affinity Capture-MS 19615732
053 FAS_DD 103 RIPK1_DD Homotypic 8838 Physical association 7538908 Affinity Capture-MS 19940151
054 IFI16_PYD 103 RIPK1_DD Heterotypic 2604 N/A N/A
055 IFIH1_CARD1 042 CRADD_DD Heterotypic 2136 N/A N/A
055 IFIH1_CARD1 058 IRAK1_DD Heterotypic 2690 N/A N/A
055 IFIH1_CARD1 071 NLRC3_CARD Homotypic 3378 N/A N/A
055 IFIH1_CARD1 115 UNC5D_DD Heterotypic 2366 N/A N/A
057 IFIH1_CARD1-CARD2 071 NLRC3_CARD Homotypic 3528 N/A N/A
059 IRAK2_DD 042 CRADD_DD Homotypic 2948 N/A N/A
059 IRAK2_DD 047 DTHD1_DD Homotypic 2184 N/A N/A
059 IRAK2_DD 062 LRRD1_DD Homotypic 2138 N/A N/A
059 IRAK2_DD 071 NLRC3_CARD Heterotypic 3100 N/A N/A
059 IRAK2_DD 115 UNC5D_DD Homotypic 3102 N/A N/A
062 LRRD1_DD 011 BCL10_CARD Heterotypic 2252 N/A N/A
065 PYRIN_PYD 009 ASC_PYD Homotypic 2938 Physical association 11498534 N/A
065 PYRIN_PYD 010 ASC_PYD-CARD 2908 Physical association 11498534 N/A
065 PYRIN_PYD 020 CARD18_CARD Heterotypic 5470 N/A N/A
065 PYRIN_PYD 021 CARD19_CARD Heterotypic 6436 N/A N/A
067 MYD88_DD 042 CRADD_DD Homotypic 3134 N/A N/A
067 MYD88_DD 047 DTHD1_DD Homotypic 2670 N/A N/A
067 MYD88_DD 115 UNC5D_DD Homotypic 3966 N/A N/A
072 NLRC4_CARD 011 BCL10_CARD Homotypic 3282 N/A Affinity Capture-MS 21907836
081 NLRP2_PYD 106 TNFRSF10B_DD Heterotypic 7216 N/A N/A
083 NLRP4_DD 004 ANK3_DD Homotypic 2058 N/A N/A
083 NLRP4_DD 011 BCL10_CARD Heterotypic 2844 N/A N/A
083 NLRP4_DD 020 CARD18_CARD Heterotypic 8070 N/A N/A
083 NLRP4_DD 021 CARD19_CARD Heterotypic 5258 N/A N/A
083 NLRP4_DD 044 DEDD_PYD Heterotypic 2948 N/A N/A
086 NLRP7_PYD 037 CFLAR_DED1 Heterotypic 6814 N/A N/A
087 NLRP8_PYD 071 NLRC3_CARD Heterotypic 2348 N/A N/A
087 NLRP8_PYD 115 UNC5D_DD Heterotypic 2460 N/A N/A
088 NLRP9_PYD 011 BCL10_CARD Heterotypic 2936 N/A N/A
088 NLRP9_PYD 020 CARD18_CARD Heterotypic 5886 N/A N/A
088 NLRP9_PYD 021 CARD19_CARD Heterotypic 4226 N/A N/A
088 NLRP9_PYD 084 NLRP5_PYD Homotypic 2852 N/A N/A
089 NOD1_CARD 071 NLRC3_CARD Homotypic 2086 N/A N/A
090 NOD2_CARD1 015 CARD10_CARD Homotypic 2438 N/A N/A
090 NOD2_CARD1 042 CRADD_DD Heterotypic 2738 N/A N/A
090 NOD2_CARD1 071 NLRC3_CARD Homotypic 3656 N/A N/A
090 NOD2_CARD1 115 UNC5D_DD Heterotypic 2200 N/A N/A
092 NOD2_CARD1-CARD2 015 CARD10_CARD Homotypic 10,988 N/A N/A
092 NOD2_CARD1-CARD2 037 CFLAR_DED1 Heterotypic 4002 N/A N/A
092 NOD2_CARD1-CARD2 042 CRADD_DD Heterotypic 2170 N/A N/A
092 NOD2_CARD1-CARD2 071 NLRC3_CARD Homotypic 8834 N/A N/A
092 NOD2_CARD1-CARD2 073 NLRC5_CARD Homotypic 3466 N/A N/A
095 PIDD1_DD 015 CARD10_CARD Heterotypic 5146 N/A N/A
095 PIDD1_DD 111 TRADD_DD Homotypic 4018 N/A N/A
097 PYDC2_PYD 011 BCL10_CARD Heterotypic 5342 N/A N/A
097 PYDC2_PYD 037 CFLAR_DED1 Heterotypic 9994 N/A N/A
097 PYDC2_PYD 042 CRADD_DD Heterotypic 4782 N/A N/A
097 PYDC2_PYD 047 DTHD1_DD Heterotypic 4984 N/A N/A
097 PYDC2_PYD 058 IRAK1_DD Heterotypic 7260 N/A N/A
097 PYDC2_PYD 062 LRRD1_DD Heterotypic 2632 N/A N/A
097 PYDC2_PYD 071 NLRC3_CARD Heterotypic 8938 N/A N/A
097 PYDC2_PYD 091 NOD2_CARD2 Heterotypic 3810 N/A N/A
097 PYDC2_PYD 105 TNFRSF10A_DD Heterotypic 3174 N/A N/A
097 PYDC2_PYD 115 UNC5D_DD Heterotypic 4210 N/A N/A
100 RIG-I_CARD1 042 CRADD_DD Heterotypic 2438 N/A N/A
100 RIG-I_CARD1 071 NLRC3_CARD Homotypic 2304 N/A N/A
100 RIG-I_CARD1 115 UNC5D_DD Heterotypic 2032 N/A N/A
101 RIG-I_CARD2 042 CRADD_DD Heterotypic 2016 N/A N/A
101 RIG-I_CARD2 058 IRAK1_DD Heterotypic 3274 N/A N/A
101 RIG-I_CARD2 071 NLRC3_CARD Homotypic 3648 N/A N/A
101 RIG-I_CARD2 091 NOD2_CARD2 Homotypic 2020 N/A N/A
101 RIG-I_CARD2 115 UNC5D_DD Heterotypic 3188 N/A N/A
102 RIG-I_CARD1-CARD2 037 CFLAR_DED1 Heterotypic 2738 N/A N/A
107 TNFRSF11B_CARD 103 RIPK1_DD Heterotypic 2722 N/A N/A
108 TNFRSF1A_DD 011 BCL10_CARD Heterotypic 4508 N/A Affinity Capture-MS 21903422
110 TNFRSF25_DD 052 FADD_DED-DD 3382 N/A N/A

Combinations were extracted where AlphaScreen signals of more than 2 × 103 RLU were observed in either FLAG × biotin or biotin × FLAG pair.