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. 2021 Feb 24;42(11):1769–1779. doi: 10.1038/s41401-021-00613-8

Fig. 4. 1,2,4-TTB had no significant effect on the activation of the AIM2 inflammasome.

Fig. 4

a–c Primary macrophages pretreated with LPS were treated with 1,2,4-TTB/Nig for 45 min or 1,2,4-TTB/poly(dA:dT) for 2 h. Then, the cells and supernatants were harvested for analysis of the expression of cleaved IL-1β and cleaved Casp-1 by Western blotting (a), and the gray values of the cleaved IL-1β (b) and cleaved Casp-1 (c) bands were analyzed with ImageJ. d Primary macrophages pretreated with LPS were treated with 1,2,4-TTB/Nig for 45 min or 1,2,4-TTB/poly(dA:dT) for 2 h, and then the supernatants were harvested for analysis of the expression of cleaved IL-1β by ELISA. e, f iBMDMs pretreated with LPS were then treated with 1,2,4-TTB/Nig for 1 h or 1,2,4-TTB/poly(dA:dT) for 2 h. Then, the cells and supernatants were harvested for analysis of the expression of cleaved IL-1β by Western blotting (e), and the gray values of the cleaved IL-1β (f) bands were analyzed with ImageJ. (* indicates P < 0.05, ** indicates P < 0.01 by two-way ANOVA).