Skip to main content
. 2021 Feb 24;42(11):1769–1779. doi: 10.1038/s41401-021-00613-8

Fig. 8. 1,2,4-TTB inhibits activation of the NLRP3 inflammasome in vivo.

Fig. 8

a Spinal cord slices from EAE model mice administered 1,2,4-TTB or vehicle were stained with an anti-ASC antibody to analyze the expression of ASC. b–d The expression of cleaved Casp-1, NLRP3, pro-IL-1β, pro-Casp-1, ASC, and β-actin was analyzed by Western blotting (b), and the gray values of the ASC (c) and cleaved Casp-1 (d) bands were analyzed by ImageJ. e–h RNA was extracted from the spinal cords of EAE model mice administered 1,2,4-TTB or vehicle, and the mRNA levels of IFN-γ (e), IL-17a (f), CCL-5 (g), and IL-4 (h) were analyzed by real-time PCR. (* indicates P < 0.05, ** indicates P < 0.01 by one-way ANOVA).