FIG. 1.

Subcellular localization of Pat1p. (A) Western blotting analysis of Pat1p, Pab1p, and Rna15p in 25 μg of proteins extracted from wild-type cells (WT) and from cells deficient in Pat1p (pat1Δ); (B) Western blotting analysis of Pat1p and Pab1p in a total extract (T), two cytoplasmic fractions (C1 and C2), and purified nucleus (N) prepared from wild-type cells. Equivalent percentages of fractions were analyzed on the gel. Nucleolar Gar1p and mitochondrial Nam1p were used as fractionation controls.