FIG. 2.

Pat1p fractionates with the 40S ribosomal subunit on a gradient. Deletion of the gene results in a decrease of heavier polysomes. Polysomes extracted from wild-type cells (WT) and from cells deficient in Pat1p (pat1Δ) and in both Pat1p and Pab1p (pat1Δ/pab1Δ) were separated on sucrose gradients. Sedimentation proceeded from left to right. 40S and 60S subunits and 80S monoribosomes are indicated by arrows on OD₂₆₀ profiles. Fractions were tested for Pat1p and Pab1p by Western blotting. The 60S ribosomal protein Ssm1p and nonpolysomal Nam1p were used as controls.