FIG. 6.

Pat1p antibodies inhibit cap- and poly(A) tail-dependent translations. Nuclease-treated extracts from wild-type cells were incubated with the indicated amounts (in micrograms) of Pab1p or Pat1p antibody. Translation was initiated by addition of LUC mRNA with the indicated structure. Luminescence was measured after 30 min of incubation at 28°C. Data are expressed as percentages of activity in the absence of antibody. These 100% values fall within the ranges indicated by the error bars shown in Fig. 5.