FIG. 8.

(A) Pat1p antibodies inhibit 40S ribosomal subunit binding to CapMFA2pA mRNA. Translation extracts from wild-type cells containing the indicated amounts of Pat1p antibodies were programmed with CapMFA2pA mRNA in the presence of cycloheximide. Initiation complexes were analyzed on sucrose gradients as described in the legend to Fig. 7A. (B) Translation extracts deficient in Pat1p are unable to form stable initiation complexes. Extracts from wild-type cells (WT) and cells deficient in Pat1p (pat1Δ) were programmed with amounts of CapMFA2pA mRNA 10 times those used in the experiments for which the results are shown in panel A. Formation of initiation complexes was analyzed as described in the legend to Fig. 7A. Ab, antibody.