Skip to main content
. 2021 Nov 3;184(24):5932–5949.e15. doi: 10.1016/j.cell.2021.10.027

Figure 2.

Figure 2

Respiratory mucosa, olfactory mucosa, and olfactory bulb in control patients

Brightfield images of hematoxylin and eosin-stained sections (top), confocal images of sections stained fluorescently with RNAscope and IHC (middle), and schematics of the main cell types with the genes (italics) and proteins (roman) used as markers (bottom).

(A) RM of control #12. FOXJ1 is a marker for ciliated cells and EPCAM for epithelial cells. The MUC5AC-IR signal labels goblet cells and identifies blobs of secreted mucus.

(B) OM of control #4. OR5A1 is one of the 389 OR genes in the human genome. OMP is a marker for mature OSNs, and KRT8 for sustentacular cells.

(C) OB of control #15. SSTR2A is a leptomeningeal marker, and TUBB3 a neuronal and axonal marker. In the schematic, the pia mater is depicted as a thin gray line surrounding the OB, the dura mater as a thick light-blue line, and the arachnoid as a brown spider web-like structure between the pia mater and the dura mater. Axons of OSNs course through holes of the cribriform plate and synapse with three second-order neurons in the OB. DAPI served as nuclear stain. The schematics show the main cell types that we studied.