Table 3.
Ordinal regression analysis on diagnostic performance for AIN3+ detection – univariable regression of the six individual markers (ASCL1, LHX8, SST, WDR17, ZIC1 and ZNF582) and multivariable regression for optimal marker panel (ZIC1 and ASCL1) and methylation‐positive detection rate at J‐threshold. LOOCV (and non‐CV for the marker panel) AUCs are reported, together with sensitivity, specificity and detection rate are for the J‐threshold. Outcome: AIN3+ (AIN3 and anal SCC) in anal tissue samples of (HIV‐negative) women and men.
| ASCL1 | LHX8 | SST | WDR17 | ZIC1 | ZNF582 | Marker panel (non‐CV) | Marker panel (LOOCV) | |
|---|---|---|---|---|---|---|---|---|
| AUC | 0.83 | 0.84 | 0.86 | 0.83 | 0.85 | 0.84 |
0.86 (95% Cl: 0.80–0.91) |
0.85 |
| Sensitivity | 81% | 75% | 90% | 82% | 84% | 81% | 85% | 84% |
| Specificity | 75% | 82% | 73% | 81% | 80% | 83% | 78% | 79% |
| J‐threshold | 0.516 | 0.574 | 0.332 | 0.544 | 0.469 | 0.560 | 0.483 | 0.525 |
| Detection rate | ||||||||
| Normal | 3/30 (10%) | 2/30 (7%) | 9/30 (30%) | 3/30 (10%) | 5/30 (17%) | 2/30 (7%) | 4/30 (13%) | 3/30 (10%) |
| AIN1 | 10/57 (18%) | 7/57 (13%) | 6/57 (11%) | 5/57 (9%) | 4/57 (7%) | 5/57 (9%) | 7/57 (12%) | 7/57 (12%) |
| AIN2 | 14/21 (67%) | 10/21 (48%) | 14/21 (67%) | 12/21 (57%) | 13/21 (62%) | 11/21 (52%) | 13/21 (62%) | 13/21 (62%) |
| AIN3 | 18/28 (64%) | 18/28 (64%) | 22/28 (79%) | 18/28 (64%) | 19/28 (68%) | 18/28 (64%) | 19/28 (68%) | 18/28 (64%) |
| SCC | 37/40 (93%) | 33/40 (83%) | 39/40 (98%) | 38/40 (95%) | 37/40 (93%) | 37/40 (93%) | 39/40 (98%) | 39/40 (98%) |