Fig. 5.

ST7 is the key mediator for MIB1‐induced pancreatic cancer cells progression. (A‐E) BxPC‐3 and SW1990 cells were infected with indicated shRNAs. Seventy‐two hours post‐infection, cells were harvested for western blotting analysis (A), RT‐qPCR analysis (B), MTS assay (C), colony formation assay (D), and transwell assay (E). For panel B‐E, data presented as mean ± SD with three replicates. Statistical significance was determined by one‐way ANOVA. *, P < 0.05; **, P < 0.01; ***, P < 0.001. For panel E, the size of the scale bar on microscopy images was 100 μm. (F and K), BxPC‐3 were infected with indicated shRNAs. Seventy‐two hours post‐infection and puromycin selection, cells were harvested for western blotting analysis (F), RT‐qPCR analysis (G), MTS assay (H), and xenograft assay (I‐K). For panel G and H, data presented as mean ± SD with three replicates (n = 3). Statistical significance was determined by one‐way ANOVA. ns, not significant; **, P < 0.01; ***, P < 0.001. The image of tumor was shown in panel I. The tumor mass was demonstrated in panel J. The tumor growth curve was indicated in panel K. Data presented as mean ± SD with five replicates (n = 5). Statistical significance was determined by one‐way ANOVA. ns, not significant; **P < 0.01;***P < 0.001.