Fig. 4.

Identification of interacting partners and co-expression network towards functional evaluation of AnxA2. A. Representative mAb150 immunoprecipitated proteins stained with Coomassie Blue; B. Correlation plot for positive correlating genes with ANXA2 gene expression in HGSC-TCGA dataset; C. Validation of predicted and immunoprecipitated AnxA2 correlating proteins by immunoblotting; D. Plot indicating correlation between EMT signature AnxA2 hub genes; E. Representative migration capabilities estimated as percent in vitro open wound area for OVCAR3, CAOV3, PEO14, A4 and OVMZ6 cells following mAb150 exposure, serum starved (denoted by ss) cells used as control; F. Representative box plots of mean migratory velocity (pixels/hr) comparison between 1: Control (Serum starved) and 2: mAb150 treated cells; G. Principal component (PC) analysis of time-lapse imaging-based migration data of control and mAb150 treated cells, filled and empty shapes indicate control and mAb150 treated cells respectively. *P<0.05; **P<0.01; ***P<0.001.