Fig. 6.

Effect of mAb150 treatment on A4 xenografts vis-à-vis drug target (AnxA2) expression and altered fold-change in the respective, sorted tumor fractions following treatment. A-i. Schematic of AnxA2^neg and AnxA2^pos cell fractions in A4 xenografts; A-ii. Regenerative hierarchy, A-iii. Ploidy, A-iv. Cell cycle fractions; B. Comparison functional assays for capability to form spheroids (B-i) and soft agar colonies (B-ii) of the regenerative hierarchies in AnxA2^neg and AnxA2^pos cell fractions; C. Heatmap representation of C-i. Spheroid formation, C-ii. adherent colony formation and C-iii. soft agar colony formation capability of residual cells in each of the PKH regenerative hierarchy fractions across 8 tumor types (1:PKH^neg; 2:PKH^lo; 3:PKH^hi). NS- Not Significant. 1: Euploid G0, 2: Aneuploid G0, 3: Euploid G1, 4: Euploid S + G2/M and Aneuploid G1, 5: Aneuploid S − G2/M. *P<0.05; **P<0.01; ***P<0.001.