Figure 1.

Morphological comparison between enlarged lysosomes observed in living condition or after different fixation procedures

HeLa cells, stably expressing the lysosomal marker Lamp1-mGFP, were treated or not (mock cells were treated with only the buffer used for the preparation of the stock solution of the amino acids analogs) for 2 h with amino acid analogs (10 mM LEU-ME) and differently handled (live versus methanol or glutaraldehyde/paraformaldehyde fixation), before being analyzed by confocal microscopy.