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. 2021 Oct 27;2(4):100916. doi: 10.1016/j.xpro.2021.100916

Figure 2.

Figure 2

Measurement of lysosomal diameter

Living HeLa cells stably expressing Lamp1-mGFP were grown in 12-multiwell plate with glass-bottom, treated or not with LEU-ME 10 mM for 2 h, and imaged by using a Ultraview VoX spinning disk microscope. Some representative lysosomal diameters are indicated by magenta lines and their lengths have been estimated by ImageJ Fiji software and shown in μm scale (A). The histogram shows the average length of lysosomal diameters from 3000 measurements from 20 cells for each of the three independent experiments (B). Statistical analysis was performed by One-way ANOVA. Scale bar: 20 μm.