FIGURE 1.

Rotational defects, including DORV was observed in 50% of Setd5 heterozygotes (Setd5 ^+/−) and VSD were observed in 75% of Setd5 ^+/− embryos at E14.5. In the WT heart, the pulmonary trunk and aorta open into separate ventricles: the right and left ventricle, respectively (a‑a‴). In Setd5 ^+/− hearts, DORV was observed. In panel B′ the aorta and pulmonary trunk are juxtaposed, and in posterior sections b‴, the aorta eventually opens into the right ventricle; both the pulmonary trunk and aorta open into the right ventricle. A perimembranous VSD is observed in Setd5 ^+/− (panel b″″). Ao, aorta; DORV, double outlet right ventricle; LA, left atrium; LV, left ventricle; PT, pulmonary trunk; RA, right atrium; RV, right ventricle; VSD, ventricular septal defects; WT, wild‐type. Scale bars = 400 μm