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. Author manuscript; available in PMC: 2022 Aug 18.
Published in final edited form as: J Am Chem Soc. 2021 Aug 4;143(32):12675–12687. doi: 10.1021/jacs.1c05386

Figure 3|. NBDY phosphorylation at T40 is required for P-body disappearance at the G2/M checkpoint.

Figure 3|

P-body disappearance during mitosis requires NBDY phosphorylation at T40. P-body numbers in nocodazole or vehicle-treated cells expressing NBDY or NBDY T40A were imaged via immunofluorescence. Yellow, anti-DCP1A immunofluorescence; blue, DAPI. Six fields of view (>180 cells) were used to quantitate average P-bodies per cell in each condition (b). Data represent mean values from each field of view ± s.e.m, and significance was evaluated with one-way ANOVA. ***P < 0.001, Dunnett’s test. Scale bars, 20 μm.