Figure 5|. P-body disappearance during EGF stimulation.

(a,b) EGF (20 ng/ml) induces P-body disassembly. Six fields of view (>180 cells) were used to quantitate average P-bodies per cell at each timepoint (b). Data represent mean ± s.e.m, and significance was evaluated with one-way ANOVA. ***P < 0.001, ****P < 0.0001, Dunnett’s test. (c) EGF concentration dependence for P-body dissociation. Six fields of view (>180 cells) were used to quantitate P-bodies per cell at each EGF concentration. Data represent mean (P-bodies per cell calculated from each field of view) ± s.e.m, and significance was evaluated with one-way ANOVA. ****P < 0.0001, Dunnett’s test.