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. Author manuscript; available in PMC: 2022 Aug 18.
Published in final edited form as: J Am Chem Soc. 2021 Aug 4;143(32):12675–12687. doi: 10.1021/jacs.1c05386

Figure 6|. NBDY phosphorylation at S61 is required for P-body disappearance during EGFR signaling.

Figure 6|

P-bodies were visualized in NBDY rescue, NBDY KO, NBDY S61A rescue and NBDY S61D rescue cell lines after treatment with EGF or vehicle. Yellow, anti-DCP1A immunofluorescence; blue, DAPI. Six fields of view (>180 cells) were used to quantitate average P-bodies per cell in untreated (b) or EGF-treated cells at indicated time points (c). Data represent mean values ± s.e.m, and significance was evaluated with one-way ANOVA. n.s., not significant; *P < 0.05; ****P < 0.0001, Dunnett’s test. Scale bars, 20 μm.