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. 2021 Oct 22;6(20):e151527. doi: 10.1172/jci.insight.151527

Figure 1. Plasma from patients with COVID-19 induces thromboinflammatory gene expression in endothelial cells.

Figure 1

HUVECs were cultured overnight in the presence of 10% pooled plasma from patients with severe (S, ICU patients), moderate (Mod, non-ICU hospitalized patients), or mild (nonhospitalized outpatients) COVID-19 or healthy controls (HC) and analyzed for relative fold mRNA expression change of tissue factor: (A) F3, (B) E selectin (SELE), (C) ANGPT2, (D) TIE2, (E) VE-PTP (PTPRB), (F) EPCR (PROCR), (G) TFPI, and (H) thrombomodulin (THBD). Where indicated, cells were pretreated with Angpt-1 (300 ng/mL) or AKB-9778 (5 μM) for 30 minutes prior to incubation with plasma (n = 3–4 individual biologic replicates performed in technical duplicate). Gene expression was normalized to that of actin and changes are shown relative to HC. Graphs represent the mean ± SD. Significance in comparison with severe (S) was determined by 1-way ANOVA using Dunnett’s post hoc test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.