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. 2021 Oct 22;6(20):e151078. doi: 10.1172/jci.insight.151078

Figure 4. Krox20-lineage tracing in the Meibomian gland.

Figure 4

(A) Lineage tracing of Krox20 with tdTomato Red reporter (Krox20-Cre R26-Tom) shows the distribution of Krox20-lineage cells in the Meibomian gland during development (<P15) and after the Meibomian gland structure is fully established (>P15). Sections are also stained with DAPI. (B) Immunofluorescence staining of the Meibomian glands from Krox20-Cre R26-Tom mice (P13) for Meibomian gland marker K14 and tdTomato (Tom). (C) Immunofluorescence staining of the Meibomian gland from Krox20-Cre R26-Tom mice (P11) for meibocyte marker PPARγ and Tom. White dashes in A–C encircle the Meibomian gland; yellow dashes in A demarcate the conjunctival surface of the eyelid; and white arrows indicate the cornea. Representative images are shown. M, months. Scale bar: 100 μm.