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. 2021 Oct 22;6(20):e151078. doi: 10.1172/jci.insight.151078

Figure 5. KROX20-positive cells are indispensable for Meibomian gland development.

Figure 5

Krox20-DTA;K14-Cre pups (P6) in which KROX20-expressing cells were deleted exhibit complete loss of Meibomian gland, as shown by coimmunostaining for (A) K14 and KROX20, (B) H&E staining, and (C) K14 and PPARγ immunostaining. (D) These mice also failed to produce meibum, as shown by the lack of Oil Red O staining in the eyelid. Dashed lines (red in B and D and white in A and C) demarcate the Meibomian glands in the control mice and its expected location in the Krox20-DTA;K14-Cre mice. n = 7 K14-Cre mice, and n = 11 Krox20-DTA;K14-Cre mice. Representative images are shown. Scale bar: 100 μm.