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. 2021 Oct 22;6(20):e151078. doi: 10.1172/jci.insight.151078

Figure 6. Inducible ablation of Krox20-lineage cells in adult mice.

Figure 6

Doxycycline-induced Krox20-lineage cell ablation from P20 to P92 causes corneal lesions. (A and B) Gross images of eyes and eyeballs from rtTA-EGFP+/–;Tet-DTA+/–;Krox20-Cre mice and controls. The black arrow in B is pointing to the corneal lesion. (C) H&E staining as well as (D) immunofluorescence staining for K14 and PPARγ and (E) K14 and KROX20 in the Meibomian glands from rtTA-EGFP+/–;Tet-DTA+/–;Krox20-Cre mice and controls. White dashed lines in C–E demarcate the Meibomian gland in the control mice and its expected location in the rtTA-EGFP+/–;Tet-DTA+/–;Krox20-Cre mice. n = 7 Krox20-DTA;K14-Cre mice, and n = 11 Krox20-DTA;K14-Cre mice. Representative images are shown. Scale bar: 100 μm.