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. 2021 Oct 22;6(20):e151078. doi: 10.1172/jci.insight.151078

Figure 7. Ablation of both KROX20 and KROX24 increases severity of dry eye.

Figure 7

(A) Gross images of the eye showing corneal lesions in Krox20fl/fl;K14-Cre (Krox20-cKO) and Krox24–/–;Krox20fl/fl;K14-Cre (DKO) mice, while Krox24–/– (Krox24-KO) and Krox20fl/fl (WT) controls maintained normal ocular surface. (B and C) H&E staining of a section of an eye showing squamous metaplasia in Krox20-cKO and DKO mice compared with Krox24-KO mice and controls. (D–G) Immunofluorescence staining of the corneas of WT, Krox24-KO, Krox20-cKO, and DKO mice for (D) corneal epithelium marker K12 and stratified epidermal markers (D–G) K14, (E) K15, (F) K1, and (G) loricrin. Gross images in A are the same as those in Figure 8A, Supplemental Figure 1 (8M), and Supplemental Figure 2A. n = 50 mice for each genotype. Among mice analyzed, 100% of Krox20-cKO and DKO mice developed corneal lesions. Representative images are shown. Scale bar: 100 μm.