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. 2021 Oct 22;6(20):e149651. doi: 10.1172/jci.insight.149651

Figure 1. TRAM deficiency alleviates atherosclerosis development.

Figure 1

Apoe−/− Tram+/+ mice and Apoe−/− Tram−/− mice were fed with HFD for 8 weeks. (A) Representative images of H&E-stained atherosclerotic lesions and quantification of plaque size demonstrated as the percentage of lesion area within aortic root area. Scale bar: 300 μm. (B) Representative images of Oil Red O–stained atherosclerotic plaques and quantification of lipid deposition within lesion area. Scale bar: 300 μm. (C) Representative images of Picrosirius red–stained atherosclerotic plaques and quantification of collagen content within lesion area. Scale bar: 100 μm. (D) Determination of total cholesterol and triglyceride levels in the plasma. (E) Determination of circulating IL-1β, TNF-α, MCP-1, and TGF-β levels by ELISA. Data are representative of 3 independent experiments, and error bars represent means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001; Student’s 2-tailed t test (n = 5 to 10 for each group).