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. 2021 Oct 22;6(20):e149651. doi: 10.1172/jci.insight.149651

Figure 3. TRAM deficiency attenuates inflammatory polarization of BMMs in vitro.

Figure 3

BM cells from WT C57 BL/6 mice and Tram−/− mice were cultured with M-CSF (10 ng/mL) in the presence of PBS or super-low-dose LPS (100 pg/mL) for 5 days. Surface expressions of CCR2 and SIRP-α on CD11b+Ly6C++ BMMs (A) and CD11b+Ly6C+ BMMs (B) were examined by flow cytometry. (C) The phosphorylation of STAT1, STAT5, and SRC in BMMs was examined by Western blotting, and relative levels were normalized to β-actin. Data are representative of 3 independent experiments, and error bars represent means ± SEM. **P < 0.01, and ***P < 0.001; 1-way ANOVA (n = 3 for each group).