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. 2021 Oct 22;6(20):e149651. doi: 10.1172/jci.insight.149651

Figure 5. TRAM-deficient resolving monocytes exhibit enhanced peroxisome homeostasis.

Figure 5

BM cells from WT C57 BL/6 mice and Tram−/− mice were cultured with M-CSF (10 ng/mL) in the presence of PBS or super-low-dose LPS (100 pg/mL) for 5 days. (A and B) Protein levels of PPARγ (A) and PEX5 (B) were examined by Western blotting, and relative expressions were normalized to β-actin. (C) BMMs were stained with anti-PMP70 and anti-LAMP1 antibodies, and the localization of peroxisomes and lysosomes was examined by confocal microcopy. Scale bars: 10 μm. Inset original magnification, 400×. (D) BMMs were labeled with CellROX, and ROS levels were quantified by flow cytometry. Data are representative of 3 independent experiments, and error bars represent means ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001; 1-way ANOVA (n = 3 for each group).