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. 2021 Oct 22;6(20):e149651. doi: 10.1172/jci.insight.149651

Figure 6. TRAM-deficient BMMs propagate its resolving nature to neighboring monocytes through CD200R.

Figure 6

(A) BMMs prepared from WT C57 BL/6 mice and Tram−/− mice, which were both CD45.2+, were transfected with CD200R siRNA or control siRNA with Lipofectamine. The CD45.2+ donor cells were then cocultured with recipient BMMs prepared from B6 SJL mice, which were CD45.1+, for 2 days. Surface expressions of CCR2, SIRP-α, and CD200R on CD45.1+ recipient BMMs were examined by flow cytometry. (B) Donor BMMs were prepared from WT C57BL/6 mice and Tram−/− mice, and recipient BMMs were prepared from B6 SJL mice. The donor cells were either directly cocultured with recipient cells or cultured in the upper chamber of a Transwell insert with recipient cells in the lower chamber. After 2 days, surface expressions of CCR2, SIRP-α, and CD200R on CD45.1+ recipient BMMs were examined by flow cytometry. Data are representative of 3 independent experiments, and error bars represent means ± SEM. **P < 0.01, and ***P < 0.001; 1-way ANOVA (n = 5 for each group).