Figure 3. Levels of FABP4 and its effect on blood glucose, insulin, and hormonal network in mouse neonates.

Plasma samples were collected from 9 WT mouse neonates within 12 hours from birth and 7 adult WT female mice. (A) FABP4 concentrations were determined using an ELISA assay and were compared between neonates and adult mice. (B) Mice heterozygous for Fabp4-null mutation were crossbred, and the offspring’s fasting blood glucose levels were tested 12 hours following birth. Levels of Fabp4^WT (n = 19), heterozygous (n = 35), and Fabp4^–/– (n = 15) mice were compared. (C) Insulin (of 11 Fabp4^WT and 11 Fabp4^–/– mouse neonates), (D) glucagon (of 3 Fabp4^WT and 4 Fabp4^–/– mouse neonates), (E) catecholamines (of 10 Fabp4^WT and 10 Fabp4^–/– mouse neonates), and (F) corticosterone (of 9 Fabp4^WT and 8 Fabp4^–/– mouse neonates) plasma levels were compared between Fabp4^WT and Fabp4^–/– mouse neonates within 12 hours following birth. Statistical analysis was performed by Student’s t test (A and C–F) and by 1-way ANOVA test (B). Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01. FABP4^WT; WT, HET; heterozygous.