Figure 6. Glycogen content of Fabp4^WT and Fabp4^–/– mouse neonates and recombinant FABP4 effect on blood glucose.

Fabp4^WT and Fabp4^–/– liver and muscle (left thigh) tissues were harvested and snap frozen at liquid nitrogen. (A and B) Glycogen content was determined in Fabp4^WT (n = 19) and Fabp4^–/– (n = 18) mouse neonatal liver (A) and Fabp4^WT (n = 5) and Fabp4^–/– (n = 5) mouse neonatal muscle (B) tissues using the Glycogen Assay Kit. (C) The ratio between blood glucose and liver glycogen content in each subject was compared between Fabp4^WT (n = 14) and Fabp4^–/– (n = 14) neonates. Recombinant FABP4 or saline was injected in Fabp4^–/– mice (n = 7 for FABP4 and 8 for saline injected) within 12 hours from delivery. (D) Blood glucose was measured from mouse tail vein at 0, 15, and 30 minutes following injections. Statistical analysis was performed by Student’s t test. Data are presented as mean ± SEM. *P < 0.05, **P < 0.01.