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. 2021 Oct 22;6(20):e145256. doi: 10.1172/jci.insight.145256

Figure 4. CEP290 localizes to the base of the primary cilium in epithelial cells.

Figure 4

(A–D) SIM images of a representative centriole and cilium for each labeling condition, CEP290, CEP164, NPHP8, and MKS3, respectively, with centrin as a marker for the BB, and AcTub as marker for the axoneme. Row average intensity plots are below each image. (E and F) Dot plot with average and standard deviation of the length distal to centrin (illustrated in intensity plot of A) or the length proximal to AcTub (illustrated in intensity plot of B). Thirty-three percent of the maximum intensity value of each channel was used as the boundary criterion for the measurement of each cilium. Measurements were compared with 1-way ANOVA and Dunnett’s post hoc test. (G) A color-coded schematic of a primary cilium with results shown. AcTub, acetylated α-tubulin.