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. 2021 Oct 22;6(20):e150813. doi: 10.1172/jci.insight.150813

Figure 4. Liver crush injury downregulates TLR2 expression in PMN in vivo.

Figure 4

(A and B) Fold change of TLR2 MFI in PMN 24 hours after S. aureus lung infection in the blood (n = 10–16/group) and the BM (n = 7–11/group). (C and D) Fold change of TLR2 MFI in PMN 4 hours after liver crush injury in the blood (n = 6–7/group) and the BM (n = 8–11/group). (E) Fold change of TLR2 MFI in PMN in BAL, 24 hours after infection with and without liver crush injury (n = 14–15/group). (F) A representative flow cytometry analysis image showing the MFI of TLR2 in PMN in BAL. Blue represents the PMN in the BAL from the “Infection” group, and red represents the PMN from the “Trauma + Infection” group. (G and H) Fold change of TLR2 MFI in PMN in the blood (n = 14–16/group) and the BM (n = 11/group), 24 hours after infection, with and without liver crush injury. (I) TLR2 mRNA expression levels in lung tissues, 24 hours after S. aureus infection with and without liver crush injury (normalized to HPRT1 mRNA expression; n = 3–4/group). All data are presented as mean ± SEM. Statistical analysis was performed by unpaired 2-tailed Student’s t test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.