Skip to main content
. 2021 Oct 22;6(20):e147033. doi: 10.1172/jci.insight.147033

Figure 3. ANT2 is necessary for the proinflammatory activation of macrophages.

Figure 3

(A) mRNA expression of inflammatory genes in WT and ANT2-MKO BMDMs before and 9 and 24 hours after LPS treatment (n = 2 wells/group). (B) The Nos2/Arg1 ratio in WT and ANT2-MKO BMDMs treated with or without PA or LPS for 24 hours (n = 2 wells/group). (C) Nitrite levels in WT and ANT2-MKO BMDM culture media, treated with or without LPS for 24 hours (n = 3 wells/group). (D) Flow cytometry analysis of the proportion of CD11c+ cells among WT and ANT2-MKO BMDMs treated with or without LPS for 24 hours (n = 3 wells/group). All values are mean ± SEM. *P < 0.05; **P < 0.01; ****P < 0.0001. NS, not significant. Statistical analysis was performed by 2-way (A) or 1-way (B–D) ANOVA with Tukey’s multiple comparison test.