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. Author manuscript; available in PMC: 2021 Nov 3.
Published in final edited form as: Cell Rep. 2021 Mar 9;34(10):108820. doi: 10.1016/j.celrep.2021.108820

Figure 2. Polq promotes the repair of 5′ DPCs in Xenopus extracts.

Figure 2.

(A) Non-denaturing gels showing a time course of DSB repair of the indicated DNA substrates in Xenopus egg extracts. % concatemer products indicated.

(B) Western blots showing the presence and absence of Polθ in mock-depleted (control) and Polθ-depleted Xenopus egg extracts. % extracts loaded indicated at right.

(C) SDS gel of purified recombinant full-length human Polθ.

(D) Non-denaturing gels showing a time course of DSB repair of the indicated DNA substrates in Xenopus egg extracts either mock depleted (control, lanes 2–5, left), Polθ depleted (lanes 6–9, left), or Polθ depleted with recombinant Polθ added (right). % concatemer products indicated.

(E) Western blots showing the presence and absence of Ku70 in mock-depleted (control) and Ku70-depleted Xenopus egg extracts. % extracts loaded indicated at right.

(F) Non-denaturing gel showing a time course of DSB repair of the indicated DNA substrate in Xenopus egg extracts either mock depleted (control, left) or Ku70 depleted (right). % concatemer products indicated.