KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| PolQ polyclonal antibody | This paper | N/A |
| PolQ polyclonal antibody | Invitrogen | PA5-69577 |
| Ku70 polyclonal antibody | Yan lab | N/A |
| Actin antibody | Invitrogen | MA-5-11869 |
| Chemicals, peptides, and recombinant proteins | ||
| Etoposide | Sigma-Aldrich | E1383 |
| Camptothecin | Sigma-Aldrich | PHL89593 |
| Formaldehyde | Sigma-Aldrich | 252549 |
| Streptavadin | Sigma-Aldrich | S3762 |
| Avidin | Sigma-Aldrich | A8706 |
| POLQ | Pometantz Lab | N/A |
| Phusion® High-Fidelity PCR Kit | New England Biolabs | E0553S |
| Lipofectamine 2000 Transfection Reagent | Thermo Fisher | 11668030 |
| Pfu DNA polymerase | Promega | M7741 |
| DpnI | New England Biolabs | R0176S |
| QIAquick PCR Purification Kit | QIAGEN | 28104 |
| Experimental models: cell lines | ||
| HCT116 | Fox Chase Cancer Center | N/A |
| HCT116 POLQ−/− | This paper | N/A |
| HCT116 TDP2−/− | This paper | N/A |
| HCT116 POLQ−/−, TDP2−/− | This paper | N/A |
| mESCs | Sfeir laboratory | N/A |
| mESCs Polq−/− | Sfeir laboratory | N/A |
| iPSCs | Sfeir laboratory | N/A |
| iPSCs Polq−/− | Sfeir laboratory | N/A |
| Polq+/+ Lin−Sca1 +c-Kit+ bone marrow cells | This paper | N/A |
| Polq−/− Lin−Sca1+c-Kit+ bone marrow cells | This paper | N/A |
| Parpl−/− Lin−Sca1+c-Kit+ bone marrow cells | This paper | N/A |
| Polq−/− Parpl−/− Lin−Sca1+c-Kit+ bone marrow cells | This paper | N/A |
| POLQ−/− HEK293T cells | This paper | N/A |
| Oligonucleotides | ||
| RP 500B | IDT | N/A |
| RP 503B | IDT | N/A |
| RP506B | IDT | N/A |
| RP507B | IDT | N/A |
| RP506PT | IDT | N/A |
| RP507PT | IDT | N/A |
| Recombinant DNA | ||
| pCMV-GFP | Addgene | 11153 |