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. 2000 May;20(10):3597–3607. doi: 10.1128/mcb.20.10.3597-3607.2000

FIG. 3.

FIG. 3

Nocodazole sensitivity of PLC1 mutants can be suppressed by NDC10 overexpression. (A) plc1Δ cells (HL1-1) are hypersensitive to nocodazole. Wild-type W303-1a (sectors 1 and 3) and plc1Δ (sectors 2 and 4) cells were streaked on a YPD plate containing 4 μg of nocodazole per ml (sectors 1 and 2; nocodazole was added to the medium from a stock solution of 3.3 mg/ml in dimethyl sulfoxide) or a YPD plate containing only a corresponding amount of the solvent (0.12% dimethyl sulfoxide; sectors 3 and 4). The plates were incubated for 3 days at 28°C. (B) Suppression of the nocodazole sensitivity of plc1-29 and plc1-39 mutants by NDC10 overexpression. plc1Δ cells (HL1-1) harboring the following plasmids were streaked on a YPD plate containing 4 μg of nocodazole per ml and incubated at 28°C for 3 days: pRS413-PLC1 and pYX242-HT-T7-NDC10 (sector 1), pRS413-PLC1 and pYX242-HT-T7 (sector 2), pRS413 and pYX242-HT-T7-NDC10 (sector 3), pRS413 and pYX242-HT-T7 (sector 4), pRS413-plc1-29 and pYX242-HT-T7-NDC10 (sector 5), pRS413-plc1-29 and pYX242-HT-T7 (sector 6), pRS413-plc1-39 and pYX242-HT-T7-NDC10 (sector 7), and pRS413-plc1-39 and pYX242-HT-T7 (sector 8).