TABLE 2.
Effect of PLC1 mutations on the mitotic stability of minichromosomes
| Straine | Generation timed | Avg loss per generation (%)a with:
|
||
|---|---|---|---|---|
| pRS314b plus nocodazole at:
|
pUN20c | |||
| 0 μg/ml | 2 μg/ml | |||
| PLC1 | 1.5 | 1.0 ± 0.1 | 1.9 ± 0.2 | 1.4 ± 0.1 |
| plc1Δ | 5.0 | 6.1 ± 0.8 | 32.4 ± 4.5 | 6.1 ± 0.6 |
| plc1-8 | 4.0 | 3.1 ± 0.4 | 12.7 ± 1.6 | 3.5 ± 0.4 |
| plc1-29 | 2.5 | 2.1 ± 0.3 | 4.1 ± 0.4 | 1.9 ± 0.2 |
| plc1-39 | 3.0 | 2.5 ± 0.3 | 5.9 ± 0.7 | 1.3 ± 0.1 |
The minichromosome stability of pRS414 or pUN20 was measured for at least five independent transformants and is reported as the percentage of minichromosome loss per generation at 28°C (standard deviations are also indicated).
The mitotic stability of the pRS413 minichromosome was measured as a fraction of cells that retained the minichromosome after growth in nonselective medium (35, 43) as described in Materials and Methods. All strains used for this assay were derived from W303-1a.
The mitotic stability of the pUN20 minichromosome was measured by using the half-sectored colony assay (27) as described in Materials and Methods. All strains used for this assay were derived from YPH499.
Generation time (in hours) was measured in YPD medium at 28°C for strains derived from W303-1a.
PLC1, plc1Δ, plc1-8, plc1-29, and plc1-39 denote plc1Δ strains harboring the pRS413-PLC1, pRS413, pRS413-plc1-8, pRS413-plc1-29, and pRS413-plc1-39 plasmids, respectively.