Figure 4.

Influence of cytidylate kinase on the phosphorylation state and lifetime of C-initiated transcripts in E. coli. (A) Effect of cytidylate kinase on the phosphorylation state of rpsT P2, efp and efp-C1A mRNA. The percentage of rpsT P2, efp, and efp-C1A 5′ ends that were monophosphorylated in an isogenic set of E. coli strains was determined by PABLO, as in Figure 1. Each value is the average of three biological replicates. Error bars correspond to standard deviations. (B) Effect of cytidylate kinase on the decay rate of rpsT P2 and efp mRNA. The decay of rpsT P2 and efp mRNA in an isogenic set of E. coli strains was monitored as a function of time after inhibiting transcription with rifampicin, plotted semilogarithmically, and analyzed by linear regression. Representative experiments are shown.