Table 1.
Structures of KEOPS proteins and sub-complexes. All structures reported are crystal structures unless stated otherwise. f = fusion protein. car-AMP = carboxy-adenylate. BK951 = TC-AMP mimetic.
| t6A modifying complex | Proteins | Species | Ligand | PDB code |
|---|---|---|---|---|
| Eukaryotes (cytoplasm) and archaea | Bud32-Cgi121 | S. cerevisiae | - | 4WWA |
| Bud32-Cgi121 | S. cerevisiae | AMP | 4WW7 | |
| Bud32-Cgi121 | S. cerevisiae | ADP | 4WW9 | |
| Bud32-Cgi121 | S. cerevisiae | AMP-PnP | 4WW9 | |
| Bud32-Cgi121 | H. sapiens | AMP-PnP | 6WQX | |
| Kae1-Bud32(f) | M. jannaschii | AMP-PnP | 2VWB | |
| Kae1-Bud32(f) | M. jannaschii | - | 3EN9 | |
| Kae1 | P. abyssi | - | 2IVO | |
| Kae1 | P. abyssi | ATP | 2IVP | |
| Kae1 | P. abyssi | AMP-PnP | 2IVN | |
| Kae1-Pcc1 | T. acidophilum, P. furiosus | - | 3ENO | |
| Kae1-Pcc1 | M. jannaschii, P. furiosus | AMP | 5JMV | |
| Kae1-Bud32(f)-Cgi121 | M. jannaschii | - | 3ENH | |
| Pcc1-Pcc1 dimer | P. furiosus | - | 3ENC | |
| Pcc1-Gon7 | S. cerevisiae | - | 4WXA | |
| Pcc1-Gon7 | S. cerevisiae | - | 4WX8 | |
| Gon7-Pcc1-Kae1 | H. sapiens | - | 6GWJ | |
| Cgi121 | S. cerevisiae | - | 4XAH | |
| Cgi121 | H. sapiens | - | 3ENP | |
| Cgi121 (NMR) | M. jannaschii | - | 2K8Y | |
| Cgi121 | M. jannaschii | tRNA | 7KJT | |
| Mitochondria | Qri7-Qri7 dimer | S. cerevisiae | 4K25 | |
| Qri7 | S. cerevisiae | AMP | 4WUH | |
| Bacteria | TsaB dimer | E. coli | - | 1OKJ |
| TsaB dimer | S. enterica | - | 2GEM, 2GEL | |
| TsaB dimer | V. parahaemolyticus | - | 3R6M | |
| TsaB dimer | P. aeruginosa | - | 4Y0W, 5BR9 | |
| TsaB dimer | T. maritima | - | 2A6A | |
| TsaE | H. influenzae | - | 1FL9 | |
| TsaE | H. influenzae | ADP | 1HTW | |
| TsaE | B. subtilus | ADP | 5MVR, 5NP9 | |
| TsaD-TsaB | E. coli | BK951, AMP | 6Z81 | |
| TsaD-TsaB | E. coli | ADP | 4YDU | |
| TsaD(E12A mutant)-TsaB | E. coli | ATP | 4WQ4 | |
| TsaD(V85E mutant)-TsaB | E. coli | ATP | 4WQ5 | |
| TsaD-TsaB | S. typhimurium | AMP | 3ZET | |
| TsaD-TsaB | S. enterica | ATP-γ-S | 3ZEU | |
| TsaD-TsaB-TsaE | T. maritima | ATP, car-AMP | 6N9A | |
| TsaD-TsaB-TsaE | T. maritima | AMPcPP | 6S84 |