Figure 1.

Binding of human LEDGF/p75 and HIV-1 IN to chromosomes spreads. Either recombinant purified LEDGF/p75 or HIV-1 IN (4nM) were incubated alone (respectively A and B) or together (C) with chromosomes spreads under the conditions described in materials and methods section. Monitoring of their interaction with the chromatin was performed by immunofluorescence with the corresponding antibody coupled to ALEXA 594 (green signal) and DAPI staining (red signal). The intensity of total IF signal has been quantified in each conditions using ImageJ software and the IN binding without or with LEDGF/p75 were reported in (D) as mean from the quantification of 7–11 chromosomes in each condition ± standard deviation (SD), P-value was calculated by student test. Scale bar = 10 μM.