Figure 3.

MavR binds the eutR transcript. (A) Predicted MavR-eutR RNA base-pairing. Point mutations to generate the disrupted alleles in the eutR^mut transcript are indicated. Numbering on the eutR sequence indicate nucleotides after the translation start site. Numbering on the MavR sequence indicate nucleotides after the transcription start site. (B) Schematic showing the in vitro transcribed RNAs used for the EMSAs. (C) RNA EMSA of labeled MavR and eutR transcripts. 9S precursor RNA is the negative control. (D) Competition RNA EMSA of labeled eutR and unlabeled MavR transcripts competed with indicated unlabeled eutR transcripts. (E) Quantification of competition RNA EMSA in (D). (F) Predicted interaction between eutR^mut and MavR transcripts.