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. 2021 Sep 30;49(19):10988–11004. doi: 10.1093/nar/gkab863

Figure 8.

Figure 8.

MavR promotes expression of genes encoding flagella. (A) RNAseq data showing flagella and motility gene expression in ΔmavR compared to WT grown aerobically. The dotted line indicates 2-fold change, N = 3. (B) RT-qPCR of flhD in WT and ΔmavR. (C) RT-qPCR of indicated genes identified as differentially expressed in the RNAseq data set in WT, ΔmavR and ΔmavR + pGEN-mavR. WT and ΔmavR carry the empty vector, N = 3. (D) Western blot of FlhD::His and FlhC::His in WT and ΔmavR. DnaK is the loading control. (E) Quantification of FlhD::His and FlhC::His expression in WT and ΔmavR,N = 3. (F) RT-qPCR of flhD/flhD::his and fliK in WT, ΔmavR and ΔmavR + pUCP24-mavR. WT and ΔmavR carry the empty vector, N = 3. (G) Quantification of WT, ΔmavR, and ΔmavR+ pGEN-mavR motility assays, N = 3. Bars represent the mean and error bars indicate SEM; ** P < 0.01, *** P < 0.001, † P < 0.1 (Student's two-sample t-test).