Figure 3.

Genetic depletion of G3BP1 and PDS treatment confer similar effects on up-regulating the protein level and down-regulating the mRNA level of PITX1. Western blot and RT-qPCR analyses for monitoring the protein and mRNA levels of PITX1 in 293T cells and the isogenic G3BP1^−/− cells (A–C), in 293T cells with or without PDS treatment (D–F), and in G3BP1^−/− cells with or without PDS treatment (G–I). Error bars represent S.D. (n = 3). The P values were calculated by unpaired, two-tailed Student's t-test. *, 0.01 ≤ P < 0.05; **, 0.001 ≤ P < 0.01; ***, P < 0.001.