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. 2021 Oct 13;49(19):10956–10974. doi: 10.1093/nar/gkab827

Figure 5.

Figure 5.

MvaT binds the ppilL2 promoter and NdpA2 increases its affinity for DNA. (A) EMSA was performed with purified MvaT (left panel) or NdpA2 (right panel) proteins, at concentrations ranging from 0 to 2000 nM, and the 500 bp ppilL2 promoter. (B) In the left panel, EMSA was performed with the 500 bp ppilL2 promoter region, 500 nM of NdpA2 and different concentrations of MvaT ranging from 0 to 2000 nM. In the right panel, EMSA was performed with the 500 bp ppilL2 promoter region, 500 nM of MvaT and different concentrations of NdpA2 ranging from 0 to 2000 nM. Retarded nucleoprotein complexes are identified by B (bound); free DNA is indicated by F (free).