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. 2021 Oct 14;49(19):10851–10867. doi: 10.1093/nar/gkab916

Figure 5.

Figure 5.

Impact of GNA nucleotide incorporation on duplex stability. (A) For the 12-mer RNA duplexes, the site of the modified base-pair is indicated by the black box (see Supplementary Table S1). Measurements were performed in 1× PBS at a duplex concentration of 2 μM and each data point is the average of two separate measurements relative to the unmodified parent. (B) The conjugate GalNAc-siRNA duplexes were modified with a single GNA nucleotide, and the site of incorporation is highlighted by the red box (g5, g6 or g7; see Supplementary Table S3). Measurements were performed on the fully modified GalNAc-siRNA duplexes (D1–D28) at a concentration of 1 μM in 0.1× PBS, and the data are represented as the average result across five different sequences relative to the parent GalNAc-siRNA not modified with GNA; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.