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. 2021 Oct 14;49(19):10851–10867. doi: 10.1093/nar/gkab916

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Evaluation of off- and on-target activity via RNA sequencing in primary mouse hepatocytes. (A) Cumulative distribution function (CDF) plots showing transcriptional dysregulation after transfection at a 10 nM dose of the indicated GalNAc-siRNAs. Each line represents the cumulative distribution of expression change among genes with or without the presence of the specified seed match in the 3′-UTR. The black line represents background genes lacking the specified seed matches below while the colored lines represent genes with at least one seed match, split by the identity of the strongest match (red = mer8, blue = mer7m8, yellow = mer7A1) (44). Delta values indicate the magnitude of each CDF shift versus background. (B) Plot of mer8 CDF shift versus on-target knockdown across various doses of the indicated GalNAc-siRNAs.