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. 2021 Oct 4;49(19):10868–10878. doi: 10.1093/nar/gkab883

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — The new Doron system subtypes provide protection against phage infection. (A) The efficiency of plaquing (EOP) for E. coli BL21-AI possessing representative Zorya type III, Hachiman type II or Lamassu type II systems from Stenotrophomonas nitritireducens DSM 12575, Sphingopyxis witflariensis DSM 14551 and Janthinobacterium agaricidamnosum DSM, respectively, relative to the empty vector control. Graphs show the mean of three biological replicates with individual data points overlaid. Two-sided t-test; * P < 0.05, ** P < 0 .01, *** P < 0.001. (B) Liquid culture infection time courses for BL21-AI strains possessing the Doron defence system variants, infected with phage PVP-SE1. Growth curves represent the mean of three biological replicates, the shaded area corresponds to the standard error of the mean.