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. 2021 Oct 4;49(19):11067–11082. doi: 10.1093/nar/gkab871

Figure 3.

Figure 3.

KRAS mutation attenuates mitotic catastrophe and relieves cell cycle arrest after radiation. (A) KRAS G13D isogenic cells were irradiated with 4 Gy. Seventy-two hours later, cells were co-stained with tubulin and DAPI to quantitate mitotic catastrophe events (multinucleated cells as shown in inserts). The graph showed percentage of mitotic catastrophe cells in minimum of 100 cells. Experiments were performed three independent times. (B) Cell cycle distribution was analyzed by BrdU incorporation assay. Left, BrdU pulsed HCT116 isogenic cells were treated with 4 Gy and collected for flow cytometry at the indicated time points. Right, proportion of cells in the G0/G1, S or G2/M phases of the cell cycle were displayed by flow cytometry histograms. Note higher proportion of cells re-entering G0/G1 at 10 h after radiation in KRAS MUT cells. Data shown as mean ± SD. Experiments were performed three times; **P < 0.001.