Figure 1.

Screening of the PKIS library. (A) Schematic representation of the screening system used to identify NMD inhibitors. HeLa cells are transfected with constructs expressing the cDNA encoding the firefly luciferase carrying MS2 binding sites in the 3′UTR and expressing an MS2/UPF1 fusion protein. If a tested molecule inhibits NMD, the firefly luciferase mRNA is stabilized and translated to a functional firefly luciferase, the activity of which is measurable. (B) Results of the screening of the two plates containing the molecules promoting the highest luciferase activity. (C) Molecules and target identification.