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. 2021 Oct 11;49(19):11022–11037. doi: 10.1093/nar/gkab882

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© The Author(s) 2021. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — AKT1 is recruited to the mRNP by UPF3X. AKT1 immunoprecipitations were performed from nuclear and cytoplasmic fractions of HEK293FT WT cells transfected with a construct expressing Globin Ter mRNA. (A) Western blot analysis of the immunoprecipitates. The three leftmost lanes of each panel of the figure correspond to serial dilutions of HEK293FT whole cell extract. (B) RNAs were purified from the immunoprecipitates in order to measure the level of Globin Ter mRNA by RT-PCR. The three leftmost lanes correspond to serial dilutions of AKT1 immunoprecipitate from HEK293FT cells transfected with Control siRNA.